Interaction of Phosphofructokinase with the Fluorescent Probe 2-(N-Methylanilino)naphthalene-6-sulphonate
نویسندگان
چکیده
منابع مشابه
Interaction of alanine with lithium. I- the alanine–Li -n (H20) n (0–2) complexes
The energy minima of systems made of an alanine molecule, a lithium cation and a various number of watermolecules have been determined with the help of quantum mechanical computations at the B3LYP DensityFunctional Theory level of computation and the standard 6-311++G (d,p) basis set. Several structures, close inenergy are found and the presence of one or two water molecules around the cation m...
متن کاملThe interaction of phosphofructokinase with erythrocyte membranes.
The interaction of muscle phosphofructokinase with human erythrocyte ghost membranes was investigated. Scatchard analysis of the binding reveals only a single class of binding sites numbering approximately 4 x 10” sites/ghost with an association constant of 2 X lo7 M-‘. Maximum binding is observed below pH 6.8 and is complete within 30 min at 4”, 24”, and 37°C; binding is inhibited by high ioni...
متن کامل1-Anilinonaphthalene-8-sulphonate, a fluorescent conformational probe for glutamate dehydrogenase.
1. The interaction of 1-anilinonaphthalene-8-sulphonate with ox liver glutamate dehydrogenase was examined. 2. The fluorescence of the dye is enhanced 100-fold on binding. 3. A further enhancement is observed when NADH and GTP are added to the enzyme. 4. By using this property of the dye to measure conformational equilibria in the enzyme the effects of coenzyme, inhibitors, enzyme concentration...
متن کاملinteraction of alanine with lithium. i- the alanine–li -n (h20) n (0–2) complexes
the energy minima of systems made of an alanine molecule, a lithium cation and a various number of watermolecules have been determined with the help of quantum mechanical computations at the b3lyp densityfunctional theory level of computation and the standard 6-311++g (d,p) basis set. several structures, close inenergy are found and the presence of one or two water molecules around the cation m...
متن کاملReaction of Cystathionase with the Fluorescent Probe
The enzyme cystathionase from rat liver is inactivated by incubation with 5,5’-dithiobis(Z-nitrobenzoic acid) at pH 7.4. The reaction of four -SH groups per mole of enzyme brings about 95% loss of the homoserine deaminase activity. Kinetically, the reactive --SH groups can be classified into two classes. The substrate L-homoserine or the competitive inhibitor L-alanine has no effect on the rate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1974
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1974.tb03345.x